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. 2011 Jan 20;6(1):e16138. doi: 10.1371/journal.pone.0016138

Figure 3. Proliferation-activating effect of microRNA mimics to miR-17, miR-20a, and miR-106b in USSC.

Figure 3

(A) USSC line SA5/03 was transfected with a negative control mimic or an equimolar batch of miR-17-, -20a-, and -106b-mimics and induced to neuronal lineage differentiation using XXL–medium 24 h after transfection. Cells were immunostained for proliferation marker Ki67 and DAPI-stained. Ki67 (green)/DAPI stains from time points 24 h and 48 h after transfection are shown from untransfected SA5/03, and from SA5/03 transfected with negative control mimic and the mimic batch, respectively. (B) Increase of Ki67 expression in native USSC as well as in XXL-USSC upon miR-17-, -20a- and -106b-mimics-batch transfection. Combined results from counting of 1500–3000 Ki67/DAPI-stained cells/experimental condition from two biological replicates with 3 technical replicates each are given, together with standard error of the means and statistical significancies (Student's t-test, unpaired: ***: p≤0.001).