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. 2011 Jan 20;6(1):e16174. doi: 10.1371/journal.pone.0016174

Figure 1. Marker expression of NTERA2 (NT2) cells and ATRA differentiated NTERA2 neurons (NT2N).

Figure 1

NT2 cells (A–C) co-express GFAP and SSEA3 with GFAP staining confined to the nucleus (A). NT2 cells also co-express GFAP and β-tubulin III (BT3) which are both mainly confined to the nucleus (B). Nestin with GFAP are co-localized in NT2 cells (C). NT2N assembled themselves into cell clusters which co-expressed GFAP and BT3 found within the cytoplasm and neurites (D). Arrow heads point to cells that appear as radial glia cells which only display GFAP staining (D). NT2N also express neural markers TAU and MAP2 with MAP2 confined to dendrites (tapering morphology shown by arrow heads) and TAU not displaying restriction to axons. In merge panel insert shows close-up view of neurite (E). Synapsin 1 staining is seen as punctate along neurite projections co-expressing BT3 (F). Photomicrographs were obtained at 100X (A–C; scale bar 200 µm) and 200X (D–F; scale bar 100 µm).