Figure 2. Cell types following all-trans-retinoic acid differentiation of NTera2 cells before purification of neurons.

Large protoplasmic astrocytes-like cells that form the basal layer of cells that NTera2 neurons rest on display weak GFAP staining (A). Neural cluster which contains multipolar fibrous astrocytes which co-express GFAP and S-100β (B). The fibrous astrocytes do not display co-expression of S-100β with β-tubulin III positive neurons (C). Photomicrographs were obtained at 100X (A; scale bar 200 µm) and 400X (B–C; scale bar 50 µm).