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. 2011 Jan 20;6(1):e16174. doi: 10.1371/journal.pone.0016174

Figure 4. Relative mRNA expression of cell marker genes and neurotransmitter related genes expressed by NTERA2 (NT2) and NTERA2 all-trans retinoic acid differentiated neurons (NT2N).

Figure 4

Relative amount of mRNA normalized to the average of 3 reference genes (G3PDH, PPIA, and NUBP1). Relative amount of mRNA for NT2 (black bar) and NT2N (gray bars) were obtained from 6 passage matched cultures (ranging from 25–36) and all samples were analyzed independently in triplicate. Panel A displays the expression of ‘stemness’, dermal layer, cell type makers, and growth factor genes. Panel B displays the relative expression of neurotransmitter related genes. All data are expressed as the average relative gene expression + SEM. For the comparison of NT2 to NT2N: # p<0.05, %, p<0.01, and & p<0.005.