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. 2011 Jan 20;6(1):e16175. doi: 10.1371/journal.pone.0016175

Figure 6. Induction of EMT in A549 cells.

Figure 6

Progression through EMT was evaluated in the adenocarcinoma-derived human lung epithelial cell line A549 72h after the addition of TGF-β1 (10 ng/mL) and EGF (50 ng/mL) to the culture medium. The epithelial junction proteins CAR and E-cadherin (A, D), the tight junction protein occludin and the mesenchymal marker vimentin (B, E) as well as the EMT-associated transcription factors pSmad3 and Snail1 (C, F) were assessed by immunofluorescent staining. Scale bar 10 µm. Quantification of the relative mRNA expression of CAR (G), occludin (H), E-cadherin (I), vimentin (J) ), α-SMA (K) and Snail1 (L) was assessed by qPCR.* p<0.05 compared to untreated cells. Data represent mean ± SEM, n = 6 from two independent experiments.