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. 2011 Jan 20;6(1):e16206. doi: 10.1371/journal.pone.0016206

Figure 2. In-cell cross-linking preserves the interaction of p120 and E-cadherin and is specific for interacting proteins.

Figure 2

(a) Western blot analysis of p120, E-cadherin, FAK, and p42/44 MAPK in p120 immunoprecipitates and lysates from A431 cells lysed in 1% digitonin or RIPA buffer following treatment with DMSO vehicle or 0.5 mM cross-linker as indicated. (b) Cadherin-negative A431-D cells, and A431-D cells stably expressing wild type (WT E-cad) or p120-uncoupled (764 E-cad) E-cadherin were prepared and analyzed as in A. (c) Western blot analysis of p120, E-cadherin, β-catenin, and α-catenin in p120, control IgG immunoprecipitates, and lysates from A431 cells treated as in panel a.