Intracerebroventricular (icv) infusion of insulin increases baroreflex gain in pregnant but not virgin rats. Results of 3-way ANOVA revealed significant (P<0.05) group and time effects, as well as group by time, treatment by time, and group by treatment by time interactions. Con, control; insulin, following 1–2 hr icv insulin infusion; aCSF, following 1–2 hr icv aCSF infusion. N are numbers in the control bars. †, P<0.05 compared to virgin. *, P<0.05, within group effect of insulin or aCSF.