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. Author manuscript; available in PMC: 2012 Feb 1.
Published in final edited form as: J Immunol. 2010 Dec 22;186(3):1554–1563. doi: 10.4049/jimmunol.1003005

Figure 2. BPANeuGc-PAA internalization in mouse primary B cells.

Figure 2

Primary murine B cells were incubated with biotinylated BPANeuGc-PAA and streptavidin-PE at 4 °C, and either (A) washed to remove unbound BPANeuGc-PAA prior to warming to 37 °C; or (B) not washed before warming to 37 °C. Shown is cell-associated BPANeuGc-PAA for acid washed cells following the initial 4 °C incubation (thin grey line); total cell associated BPANeuGc-PAA after warming to 37°C (thin black line), and internalized BPANeuGc-PAA resistant to an acid wash (thick black line). Filled grey trace represents background fluorescence of the cells with no BPANeuGc-PAA.