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. Author manuscript; available in PMC: 2012 Feb 1.
Published in final edited form as: J Immunol. 2010 Dec 22;186(3):1554–1563. doi: 10.4049/jimmunol.1003005

Figure 4. Differential pH dependence for αCD22 binding and BPCNeuAc-NP mediated αNP binding CD22.

Figure 4

CD22-Fc loaded Protein A magnetic beads were incubated with αCD22 or αNP +/− BPCNeuAc-NP at pH values 5–7 at 37 °C for three hours, then washed and analyzed by flow cytometry. Black traces are αCD22 (left panels) and αNP with BPCNeuAc-NP (right panel). Grey filled traces are isotype control (left panel) and αNP alone (right panel).