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. Author manuscript; available in PMC: 2012 Feb 1.
Published in final edited form as: J Immunol. 2010 Dec 22;186(3):1554–1563. doi: 10.4049/jimmunol.1003005

Figure 5. Mutations in the mCD22 cytosolic domain diminish internalization of αCD22.

Figure 5

mCD22−/− J2–44 murine B cell lines transfected with wild type and mutant mCD22 were tested for internalization of fluorescently labeled αCD22 antibody: (A) single and double CD22 mutations of Y843F and Y863F; and (B) single and double mutants of R737A and Q739A. Cells were incubated at 4 ° C with antibody and then washed (thin line) or not washed (dotted line) prior to the 37 °C incubation. Cells that were not washed prior to 37° C incubation were also acid-washed following the warming step to reveal internalized antibody (thick line). Cells that were acid-washed following the 4 °C incubation were included as control (filled curve).