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. Author manuscript; available in PMC: 2012 Feb 1.
Published in final edited form as: J Immunol. 2010 Dec 22;186(3):1554–1563. doi: 10.4049/jimmunol.1003005

Figure 6. αNP accumulation requires endocytosis and recycling pathways.

Figure 6

A. BPCNeuAc-NP-mediated binding and uptake of αNP (Alexa448) was monitored using BJAB cells in media alone (Untreated), in media containing 0.45 M sucrose to create hypertonicity and block clathrin-dependent endocytosis (Hypertonic Media), or in media containing Bafilomycin A to block recycling by inhibiting re-acidification of intracellular vesicles and trapping internalized CD22 in early endosomes (Bafilomycin A). Analysis was carried out as in Fig. 1, using the conditions for continuous binding and uptake. Filled grey traces are αNP without BPCNeuAc-NP, black traces are total αNP association, and red traces are internalized αNP. B. Graphical representation of the mean channel fluorescence derived from the histograms in A. Black bars represent untreated, grey bars are cells in hypertonic media, and white bars are Baf A treated cells.