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Shown are endocytosis experiments measuring increasing amounts of MESNa-resistant biotin signal. Steps A-C from Fig. 7 were carried out to measure endocytosis of CD22 in BJAB and Daudi cells, and transfected CD22 or Siglec-F in CHO cells. Western blots were developed with αCD22 (H221) for all B cell lines, and with αV5 for the CHO cell lines.
