Table 2.

Primer sets for TP53 mutation analysis and LOH analysis of the TP53 Locus

Exon	Size (bp)a	Forward primer	Reverse primer
A Nucleotide sequences of primers used for TP53 mutation analysis
Exon 4A	300	5′-CTGGTAAGGACAAGGGTTGG-3′	5′-GATGACAGGGGCCAGGAG-3′
Exon 4B	249	5′-AGATGAAGCTCCCAGAATGC-3′	5′-GATACGGCCAGGCATTGAAG-3′
Exon 5A	222	5′-TGCTGCCGTCTTCCAGTTGC-3′	5′-CTCACAACCTCCGTCATGTG-3′
Exon 5B	197	5′-CAGCTGTGGGTTGATTCCAC-3′	5′-TGAGGAATCAGAGGCCTG-3′
Exon 6	263	5′-TCAGATAGCGATGGTGAGCA-3′	5′-GCCACTGACAACCACCCTTA-3′
Exon 7	215	5′-CGCACTGGCCTCATCTTG-3′	5′-AGGGGTCAGAGGCAAGCAGA-3′
Exon 8	248	5′-GGGACAGGTAGGACCTGATTT-3′	5′-GCATAACTGCACCCTTGGTC-3′
Exon 9	232	5′-GGAGACCAAGGGTGCAGTTA-3′	5′-CCCCAATTGCAGGTAAAACA-3′
B Nucleotide sequences of primers used for LOH analysis of the TP53 Locus
D17S520	142	5′-GGAGAAAGTGATACAAGGGA-3′	5′-TAGTTAGATTAATACCCACC-3′
D17S786	167	5′-TACAGGGATAGGTAGCCGAG-3′	5′-GCTGTGAGTCCCAAAAGAGG-3′
D17S1353	122	5′-ATTCCCACTGCCACTCCTTG-3′	5′-CAGCTGAGGGATACTATTCAG-3′
D17S1566	189	5′-AAAGATCCTTATTGCCACTTTACTG-3′	5′-CTCTTACCTTGCTGGTGAGATTG-3′
D17S1866	175	5′-TGGATTCTGTAGTCCCAGG-3′	5′-GGTTCAAAGACAACTCCCC-3′

^aAmplicon sizes based on the UCSC Genome Browser (http://genome.ucsc.edu)