Table 2.
Repair of MNNG-induced DNA damage in 30 endometrial cancer patients and 30 healthy controls
| Endometrial cancer DNA damage ± SE | Control DNA damage ± SE | ||||||
|---|---|---|---|---|---|---|---|
| Number | 0 min | 120 min | P | Number | 0 min | 120 min | P |
| 1 | 24.2 ± 3.09 | 13.3 ± 0.56 | <0.05 | 1 | 15.2 ± 2.82 | 5.35 ± 0.30 | <0.05 |
| 2 | 10.2 ± 0.68 | 3.93 ± 1.16 | <0.05 | 2 | 15.4 ± 0.67 | 3.99 ± 0.62 | <0.05 |
| 3 | 5.94 ± 1.83 | 2.41 ± 0.25 | <0.05 | 3 | 17.0 ± 1.94 | 6.16 ± 0.50 | <0.05 |
| 4 | 9.94 ± 0.75 | 5.55 ± 2.32 | 0.069 | 4 | 12.7 ± 1.54 | 7.36 ± 1.80 | <0.05 |
| 5 | 16.8 ± 1.42 | 2.61 ± 0.34 | <0.05 | 5 | 14.9 ± 0.73 | 5.22 ± 2.12 | <0.05 |
| 6 | 5.46 ± 0.71 | 3.02 ± 1.20 | <0.05 | 6 | 46.3 ± 2.02 | 21.7 ± 1.08 | <0.05 |
| 7 | 36.8 ± 3.7 | 11.4 ± 0.86 | <0.05 | 7 | 31.3 ± 0.57 | 13.5 ± 1.27 | <0.05 |
| 8 | 37.4 ± 2.25 | 37.0 ± 0.58 | 0.760 | 8 | 36.5 ± 2.15 | 36.0 ± 1.54 | 0.942 |
| 9 | 15.3 ± 2.00 | 7.56 ± 0.46 | <0.05 | 9 | 21.3 ± 1.09 | 28.1 ± 3.95 | – |
| 10 | 9.85 ± 0.73 | 5.25 ± 0.55 | <0.05 | 10 | 95.9 ± 1.74 | 14.5 ± 0.82 | <0.05 |
| 11 | 9.44 ± 1.40 | 7.57 ± 2.43 | 0.083 | 11 | 26.3 ± 1.72 | 25.6 ± 2.03 | 0.853 |
| 12 | 21.3 ± 2.25 | 8.56 ± 0.63 | <0.05 | 12 | 36.8 ± 3.81 | 11.4 ± 0.89 | <0.05 |
| 13 | 27.5 ± 0.31 | 8.81 ± 0.39 | <0.05 | 13 | 10.6 ± 0.67 | 6.51 ± 1.22 | <0.05 |
| 14 | 70.0 ± 0.74 | 54.5 ± 2.90 | 0.058 | 14 | 10.5 ± 0.79 | 3.69 ± 0.98 | <0.05 |
| 15 | 17.0 ± 0.47 | 9.54 ± 0.30 | <0.05 | 15 | 13.7 ± 1.19 | 1.41 ± 0.14 | <0.05 |
| 16 | 20.7 ± 0.39 | 25.8 ± 2.26 | 0.067 | 16 | 5.68 ± 3.95 | 5.53 ± 1.75 | 0.546 |
| 17 | 45.2 ± 0.29 | 21.5 ± 0.28 | <0.05 | 17 | 6.52 ± 0.84 | 2.23 ± 3.74 | <0.05 |
| 18 | 39.0 ± 0.51 | 7.27 ± 0.21 | <0.05 | 18 | 12.5 ± 2.03 | 14.4 ± 1.31 | 0.070 |
| 19 | 10.5 ± 1.65 | 9.44 ± 1.35 | 0.453 | 19 | 33.2 ± 0.78 | 14.6 ± 0.60 | <0.05 |
| 20 | 10.5 ± 2.89 | 14.1 ± 2.04 | 0.098 | 20 | 24.3 ± 1.75 | 15.9 ± 1.53 | <0.05 |
| 21 | 9.14 ± 2.01 | 4.23 ± 0.84 | <0.05 | 21 | 32.5 ± 1.33 | 16.1 ± 0.99 | <0.05 |
| 22 | 13.3 ± 1.27 | 4.77 ± 0.83 | <0.05 | 22 | 16.9 ± 1.10 | 6.69 ± 1.34 | <0.05 |
| 23 | 8.30 ± 3.23 | 2.77 ± 0.59 | <0.05 | 23 | 14.9 ± 2.19 | 12.8 ± 0.86 | 0.084 |
| 24 | 15.2 ± 2.42 | 5.35 ± 0.45 | <0.05 | 24 | 19.9 ± 0.40 | 2.64 ± 1.97 | <0.05 |
| 25 | 9.09 ± 1.36 | 4.26 ± 0.84 | <0.05 | 25 | 7.64 ± 0.66 | 3.2 ± 2.07 | <0.05 |
| 26 | 15.7 ± 0.66 | 3.99 ± 0.66 | <0.05 | 26 | 52.7 ± 1.95 | 11.4 ± 2.23 | <0.05 |
| 27 | 15.5 ± 2.28 | 5.79 ± 1.09 | <0.05 | 27 | 7.64 ± 0.46 | 3.20 ± 0.07 | <0.05 |
| 28 | 29.4 ± 1.22 | 19.9 ± 2.58 | <0.05 | 28 | 15.5 ± 3.81 | 14.0 ± 2.35 | 0.750 |
| 29 | 17.2 ± 0.73 | 11.7 ± 1.74 | <0.05 | 29 | 12.3 ± 0.64 | 6.06 ± 0.52 | <0.05 |
| 30 | 11.0 ± 0.35 | 6.56 ± 0.45 | <0.05 | 30 | 11.9 ± 0.92 | 4.48 ± 0.53 | <0.05 |
“–” Not estimated
DNA damage for each treatment was analyzed as the percentage of DNA in the tail of 100 comets; mean ± SE. The efficacy of DNA repair was calculated by comparing the extent of DNA damage immediately and 120 min after MNNG treatment using Wilcoxon matched pairs test. The subjects with no significant repair (P > 0.05) are presented bold