Table 1.
Cytokine production of in vitro expanded leukemia-/MM-/virus-specific CD8+ T cells before and after vaccination
| After 7 days in vitro re-stimulation of CD8+ T cells | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Peptide | HIVRT476–134 | pp65495–503 | WT1126–134 | Pr3169–177 | |||||
| Day | −7 | 42 & 84 | −7 | 42 & 84 | −7 | 42 & 84 | −7 | 42 & 84 | |
| 1 | IFNg | <0.1 | <0.1 | 24.6 | 22.3 a | 0.4 | <0.1 | <0.1 | <0.1a |
| TNFa | <0.1 | <0.1 | 4.0 | 3.9 a | <0.1 | <0.1 | <0.1 | <0.1a | |
| IL2 | <0.1 | <0.1 | 0.4 | 0.2 a | <0.1 | <0.1 | <0.1 | <0.1a | |
| 2 | IFNg | <0.1 | <0.1a | 0.7 | 0.3 a | <0.1 | <0.1a | <0.1 | <0.1a |
| TNFa | n.p. | <0.1a | n.p. | 0.3 a | n.p. | <0.1a | n.p. | <0.1a | |
| IL2 | <0.1 | <0.1a | 0.2 | <0.1 | <0.1 | <0.1 | <0.1 | <0.1a | |
| 3 | IFNg | <0.1 | <0.1a | <0.1 | <0.1a | <0.1 | <0.1a | <0.1 | <0.1a |
| TNFa | n.d. | n.d. | n.d. | n.d. | n.d. | n.d. | n.d. | n.d. | |
| IL2 | <0.1 | <0.1a | <0.1 | <0.1a | <0.1 | <0.1a | <0.1 | <0.1a | |
| After 7 days in vitro re-stimulation of CD8+ T cells | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Peptide | HIVRT476–134 | pp65495–503 | MUC179–87 | MUC1153–178
MUC1138–164 |
|||||
| Day | −7 | 42 & 84 | −7 | 42 & 84 | |||||
| 9 | IFNg | <0.1 | <0.1 | <0.1 | <0.1a | <0.1 | <0.1 | <0.1 | <0.1 |
| TNFa | <0.1 | <0.1 | <0.1 | <0.1a | <0.1 | <0.1 | <0.1 | <0.1 | |
| IL2 | <0.1 | <0.1 | <0.1 | <0.1a | <0.1 | <0.1 | <0.1 | <0.1 | |
| Pre-existing | 0/4 | 2/4 | 1/4 | 0/4 | |||||
| Induction | 0/4 | 0/4 | 0/4 | 0/4 | |||||
| Loss | 0/4 | 0/4 | 1/4 | 0/4 | |||||
Intracellular cytokine production (ICC) 7 days after antigen-specific in vitro stimulation. Bold numbers indicate the specific detection of cytokine-secreting CD8+ T cells; italic numbers indicate a twofold change when compared with day −7. If for day 42 & 84 only one value is indicated then measured values are identical
n.d. not determined due to lack of material
aOnly one of the indicated time points was analyzed