. Author manuscript; available in PMC: 2011 Jan 21.

Published in final edited form as: Phytochem Rev. 2008 Oct;7(3):539–552. doi: 10.1007/s11101-008-9099-z

Table 1.

Examples of recombinant allergens produced in plants

Allergen source	Allergen	Transformation method	Host organism	Purification, tags	IgE reactivity	Comments	Reference
Betula verrucosa (pollen)	Bet v 1	TM virus	Nicotiana benthamiana	None	Sandwich ELISA	Immunogenicity tested in mice	Krebitz et al. (2000)
Malus domestica (fruit)	Mal d 2	TM virus	Nicotiana benthamiana	His-tag	Immunoblot	Anti-fungal activity	Krebitz et al. (2003)
Dermatophagoides pteronyssisnus (house dust mite)	Der p 1	Agrobacteria	Tobacco BY-2 cell culture	His-tag	T-cell proliferation	Glycosylation tested	Lienard et al. (2007)
	Der p 2				Histamine release	Secreted to culture medium
	Der p 2				IgE reactivity	Secreted to culture medium
Artemisia vulgaris	Art v 1	TM virus and Agrobacteria	Nicotiana tabacum	None	None	Secreted to the apoplast	Gadermaier et al. (2003)
Dermatophagoides farinae (house dust mite)	Der f 1	Agrobacteria	Lotus japonicus	None	None	Edible vaccine	Kato et al. (2005)
Dermatophagoides pteronyssisnus (house dust mite)	Der p 5	ZYM virus	Cucurbita pepo L. var Zucchini	His-tag	None	Edible vaccine	Hsu et al. (2004)
Cryptomera japonica (pollen)	Cry j 1	Agrobacteria	Oryza sativa	None	Oral therapy in mice	Expression in seeds	Okada et al. (2003)
	Cry j 2					Edible vaccine	Takagi et al. (2005a)
	7 Cry j 1 epitopes						Takagi et al. (2005b)