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. Author manuscript; available in PMC: 2011 Jan 21.
Published in final edited form as: Cancer Invest. 2010 Jun;28(5):544–553. doi: 10.3109/07357900903405942

Figure 3.

Figure 3

Chemotherapy causes a decrease in proliferation rather than apoptosis in newly divided cells in the neurogenic dentate gyrus. Brains were sectioned coronally at 50 µm, and coimmunohistochemistry was performed on every sixth section of the dentate gyrus for a total of approximately 8 free-floating sections per animal (n = 6–8/group) using fluorescence to identify colabeled BrdU and cleaved caspase-3 cells in the dentate gyrus. (a–f) Representative photomicrographs show the merged image of BrdU (green) and cleaved caspase-3 (red) coimmunohistochemistry. Inserts represent digitally enhanced areas of the image to specify colabeled staining. (g and h) Data are expressed as the averaged number of BrdU and caspase-3 double positive cells per dentate gyrus for each group. One-way ANOVA was performed with Dunnett’s test with saline as the control. The scale bar represents 50 µm.