Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Fluorescence emission intensity of near-IR dye-labeled oligonucleotides (■, IRD700; ▲, ZnPc 2) as a function of thermal cycle number. The reactions did not contain a target DNA or DNA polymerase. Thermal cycling conditions: 94 °C for 1 min; 55 °C for 40 s; 72 °C for 1 min. Emission wavelengths used were 700 nm for IRD700 and 690 nm for ZnPc 2. Emission intensities were normalized to the intensity secured after 5 thermal cycles.
