Appendix Table.

Supplement to Table. Cell Yield and Viability after Tooth Bud Tissue Digestion

Tooth Bud Cell Digestion and Culture over 10 Days
	Coll/Disp		% Confluence
Flask	Concentration	ICN	1	2	3	4	5	6	7	8	9	10	FCN
1	0.2 mg / 0.1 mg	350,000	Fl.	20	30	40	40	10	0	-	-	-	none
2	0.2 mg / 0.1 mg	250,000	Fl.	Fl.	5	10	10	0	-	-	-	-	none
3	0.2 mg / 0.1 mg	200,000	Fl.	10	20	10	0	-	-	-	-	-	none
4	0.2 mg / 0.1 mg	310,000	Fl.	Fl.	20	10	10	0	-	-	-	-	none
5	0.2 mg / 0.1 mg	270,000	Fl.	Fl.	10	10	20	10	0	-	-	-	none
M		276,000											none
SD		57,271											none
6	0.4 mg / 0.2 mg	350,000	Fl.	40	70	70	60	40	50	50	50	70	245,000
7	0.4 mg / 0.2 mg	450,000	Fl.	40	50	60	50	40	50	50	50	60	350,000
8	0.4 mg / 0.2 mg	320,000	Fl.	40	70	80	70	40	50	50	50	70	210,000
9	0.4 mg / 0.2 mg	380,000	Fl.	40	50	60	70	40	50	50	50	70	290,000
10	0.4 mg / 0.2 mg	430,000	Fl.	40	70	80	70	40	50	60	70	80	320,000
M		386,000											283,000
SD		54,129											56,302
11	0.8 mg / 0.4 mg	350,000	Fl.	40	30	30	30	40	50	50	50	50	140,000
12	0.8 mg / 0.4 mg	350,000	Fl.	10	10	30	30	40	50	40	30	20	100,000
13	0.8 mg / 0.4 mg	340,000	Fl.	20	10	10	20	10	10	40	30	30	130,000
14	0.8 mg / 0.4 mg	380,000	Fl.	40	40	30	30	30	50	50	30	30	120,000
15	0.8 mg / 0.4 mg	310,000	Fl.	10	10	20	10	10	20	30	40	30	90,000
M		346,000											116,000
SD		25,100											20,736

Five independently isolated 4-dpn tooth bud cell preparations were generated with each of 3 different collagenase/dispase enzyme concentrations, and grown in culture for 10 days. Initial and final cell counts were used to determine optimized enzyme concentrations of 0.4 mg/mL collagenase and 0.2 mg/mL dispase, which were subsequently used in all experimental implants. Abbreviations: Fl, floating cells; M, median; P, Wilcoxon test; SD, standard deviation; Col/Disp, collagenase/dispase; FCN, final cell number; ICN, initial cell number. N = 15, P = 0.0003.