A) In vivo glucose production is decreased in SRC-1 KO mice. Glucose production was performed on SRC-1 null and WT mice after an overnight fast (16h) in order to assess basal hepatic glucose production (n = 5 mice per group).
B-C) SRC-1 affects glucose production in primary hepatocytes. B: Measurement of glucose production in primary hepatocytes from WT or SRC-1 null animals after induction of gluconeogenesis with dexametasone and forskolin. C: Measurement of basal glucose production 48 h after overexpression of SRC-1, or as a control, GFP.
Data are represented as mean ± SEM. Unpaired student's t-test was used for evaluation of statistical significance. One asterisk indicates p < 0.05, two asterisks indicate p < 0.01 and three asterisks indicate p < 0.005.