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. Author manuscript; available in PMC: 2011 Dec 1.
Published in final edited form as: Cell Metab. 2010 Dec 1;12(6):606–618. doi: 10.1016/j.cmet.2010.11.009

Figure 3. SRC-1 controls the hepatic gluconeogenic program.

Figure 3

A) SRC-1 does not influence the glycogenolysis pathway in the liver. Liver glycogen content was measured in liver of WT and SRC-1 KO animals under fed (n=5 mice per group) or 24 hour fasted (n= 5 mice per group) conditions.

B) SRC-1 regulation of key genes in the gluconeogenic pathway in liver during the fasting adaptation. mRNA levels for the indicated genes were measured by qPCR in the liver of WT animals under fed (n=5 mice per group) and fasted (n= 5 mice per group) conditions. PEPCK: Phosphoenolpyruvate carboxykinase, cytosolic isoform; G6Pase: Glucose-6-phosphatase; FBP1: Fructose-1,6-bisphosphatase; PC: pyruvate carboxylase.

C) Hypercitrullinemia in SRC-1 KO mice. Citrulline levels in plasma of fasted (24 hours) WT and SRC-1 KO mice (n= 5 mice per group).

D) Impact of SRC-1 on important genes of the β-oxidation pathway in the liver. mRNA levels for the indicated genes were measured qPCR in the liver of WT and SRC-1 KO animals in fed (n=5 mice per group) and 24 hour-fasted mice (n= 5 mice per group). LCPT1: Carnitine palmitoyltransferase 1a, liver; MCAD: Acyl-Coenzyme A dehydrogenase, medium chain; LCAD: Acyl-Coenzyme A dehydrogenase, long chain, VLCAD: Acyl-Coenzyme A dehydrogenase, very long chain; AOX: Acyl-Coenzyme A oxidase 1.

E) mRNA levels for several important genes involved in glycolysis and lipogenesis pathways. FAS, ACC1, SCD1 (lipogenesis) and L-PK, ChREBP, Glut2, PFK1 (glycolysis) mRNA levels were measured by qPCR in the liver of SRC-1 KO and WT animals following 24 hours of fasting (n= 5 mice per group). FAS: fatty acid synthase, ACC1: acetyl-CoA carboxylase, SCD1: stearoyl-CoA desatuase 1, L-PK: pyruvate kinase, liver isoform, ChREBP: carbohydrate responsive element binding protein, Glut2: Glucose transporter 2, PFK1: phosphofructose kinase 1.

Data are represented as mean ± SEM. Unpaired student's t-test was used for evaluation of statistical significance. One asterisk indicates p < 0.05, two asterisks indicate p < 0.01 and three asterisks indicate p < 0.005.

“See also Fig.S2”