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. Author manuscript; available in PMC: 2011 Jan 21.
Published in final edited form as: Biochem J. 2009 Sep 14;423(1):79–89. doi: 10.1042/BJ20090993

Figure 4. Representative images of HFFs immunostained with antibodies against elastin (A, B and C) or fibrillin-1 (D, E and F) and HPCE cells immunostained with antibodies against fibulin-4 (I, K), elastin (J) or fibrillin-1 (L).

Figure 4

HFF cells were seeded on to glass coverslips in a 12-well plate with 1.8 × 105 cells/well and were allowed to grow for 3 days before immunostaining. Fibroblasts infected with pLK control viruses (A) produced extensive elastic fibres, whereas fibroblasts infected with fibulin-4 shRNA viruses pL335 (B) and pL337(C) produced significantly fewer elastic fibres. No statistically significant difference in the production of fibrillin-1 microfibrils was present among fibroblast cultures infected with pLK (D), pL335 (E) and pL337 (F). (G) Quantification of elastic fibres taken from at least four fields of multiple cultures represented in (A), (B) and (C). (H) Quantification of microfibrils taken from four fields of multiple cultures represented in (D), (E) and (F). **P < 0.05 compared with the respective control pLK. (I–L) HPCE cells were seeded on to glass coverslips in a 12-well plate with 4 × 105 cells/well and were allowed to grow for 7 days before immunostaining. The cultures produced significant amounts of fibulin-4 (I, K) and fibrillin-1 (L). However, no elastic fibres were detectable (J).