Table 1.
List of oligonucleotide primers used for real-time RT–PCR
| Species | Protein | Sequence (5′ → 3′) |
|---|---|---|
| Human | GAPDH-F | TCAACAGCGACACCCACTCC |
| GAPDH-R | TGAGGTCCACCACCCTGTTG | |
| Fibulin-4-F | ATGCGAGCAGCGCTGCTTCAA | |
| Fibulin-4-R | AGCCCCCATGGAAGTTGACAC | |
| Tropoelastin-F | GCTGACGCTGCTGCAGCCTA | |
| Tropoelastin-R | CAGCAAAAGCTCCACCTACA | |
| Tropoelastin pre-mRNA-F | CCAGCTACTTGGGAGGCTGA | |
| Tropoelastin pre-mRNA-R | CAGCAAAAGCTCCACCTACA | |
| LOX-F | GCAGATGTCAGAGATTATGATCA | |
| LOX-R | ATCGCCTGTGGTAGCCATAGT | |
| LOXL1-F | CAACTATGTGCAAGCATCCACT | |
| LOXL1-R: | GGTAATGCTGGTGGCAGCTGT | |
| Rat and mouse | Gapdh-F | CGGTGCTGAGTATGTCGTGGA |
| Gapdh-R | TGGCATGGACTGTGGTCATGA | |
| Rat | Fibulin-4-F | CACGGAATGCACAGATGGCTA |
| Fibulin-4-R | TGTCCACACAGCTCTCCTGTT | |
| Tropoelastin-F | TTAGGAGTCTCAACAGGTGCT | |
| Tropoelastin-R | CATTGGTATAGGGCAGTCCAT | |
| Mouse | Fibulin-4-F | CACGGAATGCACAGATGGCTA |
| Fibulin-4-R | CATCCACACAGCTCTCCTGTT | |
| Tropoelastin-F | CGGGTCTGACAGCGGTAGT | |
| Tropoelastin-R | CTCCAAGTCCTCCAGGACCT |
-F, forward; -R, reverse.