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Subcellular localization of RNASET2. HEK293 cells were transfected with myc-epitope tagged WT RNASET2 (A) and the mutated version, RNASET2 C184R (B). Immunofluorescence for endoplasmic reticulum (PDI), Golgi, and late endosome (LAMP-1) markers show partial colocalization with WT RNASET2, suggesting that RNASET2 enters the secretory pathway. The mutated version colocalized completely with the PDI, suggesting ER retention. (Scale bar: 10 μm.)
