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Synchrotron microcomputed tomography reconstructed images of the (A) WT, (B) tasA mutant, and (C) epsH mutant of B. subtilis biofilms following atomic layer deposition (ALD) of heavy metal oxides by vapor exposure (see Materials and Methods). In the wild-type biofilm, the vapor-phase ALD precursors penetrate only a short distance. In tasA and particularly the eps-deficient colony, deep gas penetration was observed.
