FIGURE 1.
T-type calcium channel isoforms Cav3.1 and Cav3.2 associate with Cav-3. Neonatal mouse myocyte homogenates were subjected to immunoprecipitation with either anti-Cav3.1 or Cav3.2 or Cav-3 antibodies, and the immunoprecipitates were analyzed by immunoblotting. A, representative immunoblot showing Cav3.1 and Cav-3 detected in the immunoprecipitates with either of the two antibodies, whereas control IgG does not immunoprecipitate the proteins. B, representative immunoblot showing Cav3.2 and Cav-3 detected in the immunoprecipitates with either of the two antibodies, whereas control IgG does not immunoprecipitate the proteins. C, representative immunoblot showing co-immunoprecipitation of Cav3.1 and Cav3.2 proteins from transiently expressed HEK293 cell lysates using anti-Cav-3 antibody. Both Cav3.1 and Cav3.2 channel proteins co-immunoprecipitate with anti Cav-3 antibody, whereas control IgG does not immunoprecipitate either protein. Signal for Cav3.1 and Cav3.2 channel proteins was not detected in the untransfected HEK293 cell lysates. Results are representative of 4 different experiments.