FIGURE 6.
Cav-3 inhibits ICav3.2 in mouse neonatal ventricular myocytes. A, average peak ICa,T density in neonatal ventricular myocytes infected with AdGFP control or with either AdCav3.2 or AdCav3.2+Cav-3 as indicated in the bar plot. Cav-3 overexpression significantly inhibited the AdCav3.2-mediated increased ICav3.2. B, average densities of ICa,L in neonatal ventricular myocytes infected with adenovirus as indicated. Adenovirus infection did not alter the average ICa,L density in the cells. The data represent mean ± S.E. (n = 3–5 cells, *, p > 0.005 with respect to AdCav3.2). Whole cell patch clamp analysis on the effect of siRNA-mediated knockdown of Cav-3 on ICa,T in mouse neonatal ventricular myocytes. C, average peak ICa,T density from nontransfected control (NT), GFP control, siRNA to Cav-3 or siRNA to GAPDH (control)-transfected cells as indicated in the bar plot. Representative corresponding T-type current traces are shown above. D, average densities of ICa,L in neonatal ventricular myocytes transfected as above and representative corresponding ICa,L current traces are shown above the bar plot. The average peak ICa,T or ICa,L was not significantly different and the data represent mean ± S.E., number of cells used are indicated in parentheses.