FIGURE 5.

Absence of the replication fork associated factors (RRM3, TOF1, CSM3, and DIA2) lead to karyotypic instability. A, ethidium bromide strained pulse field agarose gel showing karyotype changes in uninduced WT, tof1Δ, rrm3Δ, rrm3Δtof1Δ, csm3Δ, and rrm3Δcsm3Δ strains; B, same as in A, excepting that the cultures were induced for Ty1 retrotransposition by growing at 20 °C for 144 h; the un-induced culture of tof1Δ, rrm3Δ, and rrm3Δtof1Δ, csm3Δ and rrm3Δcsm3Δ strains showed size variations of different chromosomes as described in the text; C and D, panels showing the karyotype changes of WT, tof1Δ, dia2Δ, dia2Δtof1Δ, csm3Δ, and dia2Δcsm3Δ strains in uninduced and induced cultures, respectively; the dia2Δ, dia2Δtof1Δ and dia2Δcsm3Δ cells showed size variations in different chromosomes that are described in the text.