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. 1973 Aug;52(8):1925–1930. doi: 10.1172/JCI107376

Lymphocyte Transformation during Dinitrochlorobenzene Contact Sensitization. AN IN VITRO AND IN VIVO EVALUATION OF THE PRIMARY IMMUNE RESPONSE IN MAN

A Edgar Miller 1, William R Levis 1
PMCID: PMC302474  PMID: 4719669

Abstract

An evaluation of cell-mediated immunity in man is described that combines the advantages of an in vitro technique, lymphocyte transformation, with the use of contact sensitization to a primary immunogen, dinitrochlorobenzene (DNCB). DNCB, when coupled to autologous or allogeneic peripheral blood leukocytes, forms a complex, DNCB-antigen, that induces lymphocyte transformation specifically in leukocyte cultures from subjects sensitized to DNCB. Sequential studies of lymphocyte transformation to DNCB-antigen show that specifically reactive lymphocytes are first detected at about 10 days after in vivo application of a sensitizing dose of DNCB and reach a peak at about 14-21 days.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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