FIGURE 3.

Effect of sPLA₂-IIA on AA mobilization in RA FLSs. Human synovial FLSs were labeled with [³H]AA and stimulated (A) with (closed circles) or without (open circles) BK (10 nm) or (B, C) as indicated in the presence or absence of pyrrophenone (Pyr) for 2 h prior to harvesting medium and cells for AA release determination as described under “Experimental Procedures.” Data are mean ± S.E. of two independent experiments combined, each comprising duplicate determinations from four independent cultures. *, p < 0.05; **, p < 0.05; ***, p < 0.001 (Student's paired t test) relative to control unless indicated. Total radioactivity incorporated into cells ranged from 8,185–38,367 dpm.