FIGURE 3.

Overexpression of MyoD/EnR in P19 cells inhibited the expression of premyogenic mesoderm factors. P19(Control) and P19(MyoD/EnR) cells were differentiated in the presence of 0.8% DMSO. Total RNA was harvested on day 0 and 6 or 9. I, Northern blots with 12 μg of RNA were probed with the factors indicated on the right. II, RNA was analyzed using Q-PCR. The data have been normalized to the expression of β-actin and are expressed relative to day 0 P19(Control) cells. Error bars represent the average ± S.E. of two independent differentiations of two independent clones.