FIGURE 4.

MyoD binds directly to premyogenic mesoderm transcription factors. P19(Control) and P19(MyoD) stable cell lines were differentiated in the absence of DMSO, and ChIP was performed using an anti-MyoD antibody to identify enriched MyoD targets in P19(MyoD) cells on day 0, 4, and 6 of differentiation. Q-PCR was used to analyze the chromatin isolated for the genes indicated. Error bars represent the average ± S.E. (n = 3; *, p < 0.05). Statistical analysis was performed using the Student's t test versus IgG.