FIGURE 5.

Northern analysis of pre-rRNA processing in strains containing Dim2 mutants. A, Nob1TAP; Dim2::Gal strains supplemented with plasmids encoding wild type or mutant Dim2 under the constitutive TEF promoter were grown in glucose-containing medium for 0, 8, or 12 h prior to harvest. Total RNA was extracted and separated on an agarose-formaldehyde gel. The gel was transferred onto a membrane and probed with the indicated oligonucleotides. The sequences and locations of these probes are listed in Table 1. B, accumulation of 20 S, 21 S, and 23 S pre-rRNAs after 8 h in glucose relative to the strain with plasmid-encoded wild type Dim2 (adjusted for loading differences by normalization to the U2 probe). These averages are obtained from three or more independent experiments.