TABLE 4.
Femoral histomorphometry and bone microarchitecture of 1-, 3-, and 6-month-old Oc-Cre/+;Hes1Δ/Δ;Hes3−/−;Hes5−/− (Hes1/3/5 null) male mice and Hes1loxP/loxP;Hes3−/−;Hes5−/− (Control) controls
Femoral histomorphometry and microcomputed tomography were performed on femurs from 1-, 3-, and 6-month-old Oc-Cre/+;Hes1Δ/Δ;Hes3−/−;Hes5−/− male mice and Hes1loxP/loxP;Hes3−/−;Hes5−/− male littermate controls. Values are means ± S.E.; n = 5–12.
| Males | 1-month-old |
3-month-old |
6-month-old |
|||
|---|---|---|---|---|---|---|
| Control | Hes1/3/5 null | Control | Hes1/3/5 null | Control | Hes1/3/5 null | |
| Femoral histomorphometry | ||||||
| Bone volume/tissue volume (%) | 9.5 ± 2.0 | 16.7 ± 2.2a | 18.2 ± 1.3 | 25.1 ± 0.9a | 16.4 ± 1.2 | 27.9 ± 4.0a |
| Trabecular separation (μm) | 282 ± 98 | 142 ± 26 | 135 ± 8 | 97 ± 5a | 175 ± 9 | 115 ± 18a |
| Trabecular no. (mm−1) | 4.1 ± 0.8 | 6.5 ± 0.9 | 6.1 ± 0.3 | 7.8 ± 0.3a | 4.9 ± 0.2 | 6.8 ± 0.6a |
| Trabecular thickness (μm) | 22.9 ± 0.7 | 25.8 ± 0.6a | 29.9 ± 1.1 | 32.5 ± 1.0 | 33.5 ± 1.8 | 40.1 ± 4.0 |
| Osteoblast surface/bone surface (%) | 14.8 ± 1.7 | 18.7 ± 4.0 | 6.5 ± 0.4 | 6.3 ± 0.8 | 9.2 ± 0.6 | 8.3 ± 1.4 |
| No. of osteoblasts/bone perimeter (mm−1) | 13.8 ± 1.5 | 17.0 ± 3.9 | 6.7 ± 0.4 | 6.5 ± 0.7 | 9.7 ± 0.7 | 8.9 ± 1.4 |
| Osteoid surface/bone surface (%) | 1.88 ± 0.40 | 1.08 ± 0.33 | 0.17 ± 0.07 | 0.20 ± 0.07 | 0.16 ± 0.05 | 0.07 ± 0.03 |
| Osteoclast surface/bone surface (%) | 12.0 ± 1.0 | 10.0 ± 0.6 | 7.5 ± 0.6 | 7.2 ± 0.5 | 9.4 ± 0.5 | 8.5 ± 0.6 |
| No. of osteoclasts/bone perimeter (mm−1) | 5.8 ± 0.5 | 4.6 ± 0.3a | 3.4 ± 0.2 | 3.2 ± 0.2 | 4.0 ± 0.2 | 3.7 ± 0.2 |
| Eroded surface/bone surface (%) | 25.0 ± 1.9 | 20.6 ± 1.4 | 14.6 ± 1.1 | 13.6 ± 0.8 | 16.3 ± 0.9 | 15.3 ± 0.9 |
| Mineral apposition rate (μm day−1) | 1.44 ± 0.09 | 1.95 ± 0.10a | 0.62 ± 0.04 | 0.50 ± 0.04a | 0.50 ± 0.06 | 0.49 ± 0.03 |
| Mineralizing surface/bone surface (%) | 3.1 ± 0.2 | 2.4 ± 0.5 | 4.6 ± 1.0 | 2.8 ± 0.6 | 5.0 ± 1.5 | 5.1 ± 1.3 |
| Bone formation rate (μm2 μm−3 day−1) | 0.045 ± 0.006 | 0.043 ± 0.006 | 0.031 ± 0.007 | 0.014 ± 0.004a | 0.030 ± 0.010 | 0.026 ± 0.007 |
| Microcomputed tomography | ||||||
| Bone volume fraction (%) | 10.8 ± 1.9 | 16.7 ± 1.8a | 16.5 ± 0.9 | 26.0 ± 1.5a | 14.6 ± 2.8 | 26.9 ± 1.8a |
| Trabecular separation (μm) | 341 ± 67 | 195 ± 16 | 176 ± 8 | 137 ± 6a | 222 ± 17 | 155 ± 7a |
| Trabecular no. (mm−1) | 3.6 ± 0.6 | 5.3 ± 0.4a | 5.4 ± 0.2 | 6.5 ± 0.2a | 4.4 ± 0.4 | 5.8 ± 0.2a |
| Trabecular thickness (μm) | 40 ± 1 | 41 ± 1 | 43 ± 1 | 46 ± 2 | 50 ± 2 | 53 ± 3 |
| Connectivity density (mm−3) | 200 ± 49 | 361 ± 45a | 247 ± 22 | 385 ± 30a | 149 ± 25 | 273 ± 14a |
| Structure model index | 1.87 ± 0.05 | 1.73 ± 0.14 | 1.7 ± 0.1 | 0.9 ± 0.2a | 1.97 ± 0.18 | 0.80 ± 0.29a |
| Cortical thickness (μm) | 118 ± 6 | 115 ± 4 | 223 ± 11 | 212 ± 4 | 229 ± 13 | 188 ± 11a |
a Significantly different from wild type controls, p < 0.05.