TABLE 1.

NMR and refinement statistics for the solution structure of FL-MANF

NMR distance and dihedral constraints
Distance constraints
Total NOE	3090
Intra-residue	772
Inter-residue
Sequential (|i − j| = 1)	761
Medium range (|i − j| ≤ 4)	950
Long range (|i − j| ≥ 5)	607
Total dihedral angle restraints (used for the program Cyana)	172
φ	86
ψ	86
Structure statistics
Violations (mean and S.D.)
Distance constraints (Å)	0.009 ± 0.0003
Maximum distance constraint violation (Å)	0.29
Dihedral angle constraints (°)
Maximum dihedral angle violation (°)
Deviations from idealized geometry
Bond lengths (Å)	0.0104 ± 0.0001
Bond angles	2.1999 ± 0.0170°
Average pairwise r.m.s.d. (Å)a
Backbone	0.472 (0.346)
Heavy	0.819 (0.762)

^a Pairwise r.m.s.d. was calculated among 15 refined structures for residues 7–91 (112–147).