FIGURE 2.

Effect of interaction between Cdc42L61 and PAK2 on PAK2-catalyzed reaction. A, time courses of PAK2-catalyzed reactions. The reaction mixture contained standard assay buffer mixture and 200 μm MLCtide. The reactions were initiated by adding 3 nm phosphorylated PAK2 (trace 1) and 7.5 nm unphosphorylated PAK2 (trace 2). No significant enzyme activity was observed upon addition of unphosphorylated PAK2 in the absence of Cdc42L61. Following addition of 3 nm phosporylated PAK2 (as the arrow indicated), a linear increase in absorbance at 340 nm occurred (trace 3). B, dependence of the initial rate of the PAK2-catalyzed reaction on Cdc42L61 concentration. The reaction mixture contained standard assay buffer mixture, 200 μm MLCtide and different concentrations of Cdc42L61. The reaction was initiated by addition of 3 nm phosphorylated PAK2 into the assay system.