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. 2010 Nov 22;286(4):2689–2695. doi: 10.1074/jbc.M110.156505

FIGURE 6.

FIGURE 6.

Effect of MLCtide concentration on PAK2 autoactivation. A, plot of the steady-state velocities vs against substrate concentration, [S]. The solid line represents the best fitting result according to Michealis-Menten equation with KS = 292 μm, k2 = 34 s−1. B, plot of the apparent second-order rate constant A against substrate concentration, [S]. The continuous line represents the best fitting result according to Equation 5 with k+0 = 14.4 μm−1 s−1, and a fixed KS = 292 μm. C, plot of the reciprocal of the apparent second-order rate constant, A, against substrate concentration, [S]. D, plot of the intra-initiation rate constant ke against substrate concentration, [S]. The solid line represents the best fitting result with ke of 0.0254 ± 0.0007 s−1.