Skip to main content
. 2010 Nov 18;286(4):2807–2816. doi: 10.1074/jbc.M110.161596

FIGURE 2.

FIGURE 2.

Exonuclease activity of TTHA0118, Mpn140, NrnA, ttRecJ, and cd-ttRecJ. An aliquot of 10 nm 5′-32P-labeled 11-mer ssDNA (11f) was reacted with 0.1 or 1 μm TTHA0118 (tt0118), Mpn140, NrnA, ttRecJ, or cd-ttRecJ for 1 h at 37 °C. The reaction mixtures contained 50 mm HEPES, 100 mm KCl, 5 mm MnCl2 (for TTHA0118, Mpn140, and NrnA reactions) or 5 mm MgCl2 (for ttRecJ and cd-ttRecJ reactions) (pH 7.5). Lane 1, 5 mm MnCl2; lane 2, 5 mm MgCl2; lane 3, 0.1 μm TTHA0118; lane 4, 1 μm TTHA0118; lane 5, 0.1 μm Mpn140; lane 6, 1 μm Mpn140; lane 7, 0.1 μm NrnA; lane 8, 1 μm NrnA; lane 9, 0.1 μm ttRecJ; lane 10, 1 μm ttRecJ; lane 11, 0.1 μm cd-ttRecJ; lane 12, 1 μm cd-ttRecJ. The 11-mer arrow indicates the position of 5′-end labeled substrate 11-mer ssDNA, and the 1-mer arrows indicate the position of released 5′-end-labeled mononucleotides in control reactions with buffer containing Mn2+ or Mg2+ (lanes 1 and 2). Authentic 5′-end-labeled AMP was used as a marker for the position of mononucleotides.