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Chloride dependence of mutants of the other putative chloride site residues. HeLa cells expressing WT and the indicated mutants of GAT-1 were assayed for [3H]GABA transport as described under “Experimental Procedures” and in Fig. 3. The data shown at the indicated chloride concentrations are normalized to those of GAT-1-WT at 150 mm chloride (no gluconate substitution) and are the means ± S.E. of at least three separate experiments performed in quadruplicate.
