TABLE 1.
Cell surface expression of GAT-1 mutants
The bands were scanned after exposure of the films for 30 s. Scanning and quantification of the bands on the developed films was done as described under “Experimental Procedures.” The response for the bands with medium intensity was linear up to 2 min. The sum of the intensities of the monomer and the dimer bands were normalized against the corresponding value for GAT-1-WT (percentage of GAT-1-WT ± S.E.).
| Intensity | n | |
|---|---|---|
| GAT-1-WT | 100 | |
| S331E | 110 ± 8 | 3 |
| Q291E | 89 ± 23 | 8 |
| Q291E/S331E | 74 ± 17 | 6 |
| Q291N | 100 ± 10 | 9 |
| Q291N/S331E | 110 ± 11 | 7 |
| Q291S | 71 ± 8 | 8 |
| Q291S/S331E | 143 ± 21 | 8 |
| Q291G | 111 ± 16 | 8 |
| Q291G/S331E | 121 ± 10 | 6 |
| Q291C | 94 ± 19 | 6 |
| Q291C/S331E | 85 ± 20 | 4 |
| Q291A | 20 ± 9 | 7 |
| Q291A/S331E | 80 ± 18 | 6 |
| Y86F | 133 ± 16 | 4 |
| S295C | 157 ± 28 | 4 |
| N327Q | 138 ± 26 | 4 |
| S331A | 113 ± 30 | 5 |
| S331G | 82 ± 15 | 5 |