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The αTub410C peptide is not structured in aqueous solution. A, assigned 1H-15N HSQC of the αTub410C peptide in 20 mm MES-KOH, pH 6.9, at T = 20 °C. All amide protons were assigned. B, secondary chemical shifts of the αTub410C peptide in 20 mm MES-KOH, pH 6.9, at T = 20 °C. 13Cα, 13C′, and Hα are presented.
