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Calcium opposes the promotion of microtubule assembly by tubulin cationic partners. 30 μm tubulin polymerization was monitored turbidimetrically at 350 nm at 37 °C in 20 mm MES-KOH, pH 6.9, 2 mm MgCl2, and 1 mm GTP with 25% glycerol (panels A and B). Tau, spermine, and calcium were added at the concentrations indicated in the figure.
