FIGURE 2.

The p27RF-Rho-Rho axis is required for melanoma cell metastasis in vivo. a, the efficiency of knockdown of p27RF-Rho (sh-p27RF1 and sh-p27RF2), RhoA (sh-RhoA1, 2), or RhoC (sh-RhoC1, 2) in B16F10 cells was analyzed by Western blot analysis. b, appearance of murine lungs 14 days following injection of control or p27RF-Rho-, RhoA-, or RhoC-depleted cells into the tail veins of 7–8-week-old mice. The number of surface tumor nodules is shown in c. The data represent the means ± standard deviation (n = 7).