FIGURE 2.

Incorporation TFT into DNA by several DNA polymerases. Actions of TFT and BrdU on DNA primer extension in vitro. The ability of pfu DNA polymerase (pfu, 2.5 U/10μ), Sequenase DNA polymerase (Sequenase, 13 U/10μ), and Klenow fragment DNA polymerase (Klenow, 2.0 U/10μ) to extend the 5′-³²P-labeled primer/M13mp19(+) DNA (5 μg/mL) was evaluated in the presence of dATP, dGTP, and dCTP with or without dTTP, with TFT-TP, and with BrdUTP. Lanes 14, dideoxynucleotide sequencing of the M13mp19(+) DNA template with ddGTP, ddATP, ddTTP, and ddCTP, respectively, are indicated.