Table 2.
Fluorescence intensity analysis after PNGase F treatment
| PANEL A | CTR 1 | AD 1 | ||||||
|---|---|---|---|---|---|---|---|---|
| Band | Native 1 1 h | Native 3 3 h | Denatured 1 h | Denatured 3 h | Native 1 1 h | Native 3 3 h | Denatured 1 h | Denatured 3 h |
| 1 | 12271 (41.2%) |
11658 (38.6%) |
8608 (57%) |
978 (5.5%) |
20528 (40.5%) |
16066 (37.4%) |
10821 (30.5%) |
10480 (25.3%) |
| 2 | 5297 (17.8%) |
5272 (17.4%) |
4313 (28%) |
9264 (52.6%) |
10135 (20%) |
5651 (13.1%) |
12959 (36.6%) |
15418 (37.1%) |
| 3 | 12229 (41%) |
13257 (44%) |
2240 (15%) |
4689 (26.6%) |
19972 (39.5%) |
21315 (49.5%) |
11668 (32.9%) |
15594 (37.6%) |
| 4 | 2702 (15.3%) |
|||||||
| PANEL B | CTR 2 | AD 2 | ||||||
| Band | Native 1 1 h | Native 3 3 h | Denatured 1 h | Denatured 3 h | Native 1 1 h | Native 3 3 h | Denatured 1 h | Denatured 3 h |
| 1 | 18537 (46.7%) |
12721 (44.9%) |
6925 (25.1%) |
5234 (15.7%) |
10371 (44.4%) |
10937 (37.2%) |
6649 (37.1%) |
5579 (36.7%) |
| 2 | 6293 (15.9%) |
2825 (10%) |
11136 (40.4%) |
14381 (43.3%) |
2916 (12.5%) |
4048 (13.8%) |
5527 (30.8%) |
3913 (25.8%) |
| 3 | 14817 (37.4%) |
12804 (45.1%) |
6094 (22.1%) |
10005 (30.1%) |
10083 (43.1%) |
14385 (49%) |
5759 (32.1%) |
5706 (37.5%) |
| 4 | 3428 (12.4%) |
3632 (10.9%) |
||||||
Band fluorescence intensity after PNGase F treatment (see Western Blot bands in figure 1, panels A and B).