Figure 3. NF-κB and PKCζ are phosphorylated in response to activation of MOR and are blocked by MG132 and a PKCζ PSI.

HEK-293-MOR cells were pretreated for 45 min with 50 μM MG132, 50 μg/ml CHX, or 25 μM PKCζ PSI, followed by 100 nM DAMGO treatment. At the appropriate time following DAMGO treatment, whole cell lysates were prepared and subjected to Western blot analysis of total p65, phospho (P)-p65 (Ser536 and Ser311), and GAPDH (A) or phospho-PKCζ, total PKCζ, or GAPDH (B). This experiment is representative of three independent experiments.