Figure 5. Activation of NF-κB transcriptional activity following MOR activation.

HEK-293-MOR cells were transiently transfected with the phosphorylated NF-κB-SEAP construct prior to NF-κB SEAP reporter analysis in the absence (A) or presence of the PKCζ PSI (B), which was added at 25 μM for 45 min prior to DAMGO treatment at 0, 1, or 100 nM, and cell supernatants were collected at the designated time-points for SEAP assay. Values are a mean (±sd) of four replicates and are representative of three independent experiments.