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. Author manuscript; available in PMC: 2011 Jan 21.
Published in final edited form as: J Biol Chem. 2008 Jan 7;283(10):6145–6153. doi: 10.1074/jbc.M707981200

FIGURE 1. Immunoblot analysis of yeast expressing RTA or E177K either with (A and B) or without (C and D) its signal sequence.

FIGURE 1

Cells were grown for the hours indicated. 10 μg of fractionated supernatant (S18) and a corresponding volume of fractionated membranes (P18) were separated on SDS-PAGE. Blots were initially probed with polyclonal anti-RTA and subsequently stripped and reprobed with anti-Dpm1p and anti-Pgk1p. Cells expressing RTA (E), E177K (F), mature RTA (G), or mature E177K (H) were grown for the hours indicated in the presence or absence of 1 μg/ml tunicamycin (Tm). Unfractionated total extract (10 μg) was separated on a SDS-polyacrylamide gel. Blots were initially probed with polyclonal anti-RTA and subsequently stripped and reprobed with anti-Dpm1p. Cells harboring the empty vector were analyzed as indicated.