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. 2011 Jan 6;4:1. doi: 10.1186/1755-1536-4-1

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Copyright ©2011 Ruehl et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (<url>http://creativecommons.org/licenses/by/2.0</url>), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Activation and activity of pro/actMMP-2 in the presence of (GPO)₁₀and immobilized CI. (A) Activation of proMMP-2 (50 ng) or (B) activity of fully activated actMMP-2 alone or mixed with 10-fold molar excesses of (GPO)₁₀was determined in CI- or bovine serum albumin (BSA)-coated wells with 800 nM MCA-Pro-Leu-Gly-Leu-Dnp-Dap-Ala-Arg-NH₂substrate. Conversion of the fluorogenic substrate was monitored over time (data collection rate min^-1) at 37°C. Graphs represent mean values of three independent experiments.