Figure 2.

In vitro cytotoxicity of cisplatin. Cells (5 × 10⁴/well) were seeded in 96-well culture plates for 72 hours, then treated with cisplatin in serum-free Ham medium. Treatment conditions were: 1 hour at 37°C (dark triangles), 1 hour at 42°C (open triangles), 2 hours at 37°C without (dark squares) or with (clear squares) 2 mg/l adrenaline. Mean and SD of 4 determinations of cell survival (percent of control cells) are represented.